Kev kho mob ntawm tes autologous uas siv platelet-nplua nuj plasma (PRP) tuaj yeem ua lub luag haujlwm pabcuam hauv ntau yam kev kho mob kho mob.Muaj kev xav tau thoob ntiaj teb rau cov tswv yim kho cov ntaub so ntswg rau kev kho cov neeg mob musculoskeletal (MSK) thiab cov kab mob txha caj qaum, osteoarthritis (OA) thiab cov mob ntev thiab cov qhov txhab refractory.PRP txoj kev kho yog ua raws li qhov tseeb tias platelet loj hlob zoo (PGF) txhawb kev kho qhov txhab thiab kho qhov txhab (mob, kev loj hlob thiab kho dua tshiab).Ntau qhov sib txawv PRP formulations tau raug soj ntsuam los ntawm tib neeg, hauv vitro thiab tsiaj kev tshawb fawb.Txawm li cas los xij, cov lus pom zoo ntawm kev tshawb fawb hauv vitro thiab tsiaj txhu feem ntau ua rau cov txiaj ntsig sib txawv, vim tias nws nyuaj rau kev txhais cov kev tshawb fawb tsis zoo thiab cov lus pom zoo rau hauv tib neeg kev kho mob.Nyob rau hauv xyoo tas los no, kev vam meej tau ua tiav hauv kev nkag siab txog lub tswv yim ntawm PRP thev naus laus zis thiab cov tshuaj lom neeg, thiab cov lus qhia tshiab tshawb fawb thiab cov lus qhia tshiab tau raug npaj.Hauv qhov kev tshuaj xyuas no, peb yuav tham txog qhov kev nce qib tshiab tshaj plaws hauv kev npaj thiab muaj pes tsawg leeg ntawm PRP, suav nrog cov koob tshuaj platelet, leukocyte kev ua haujlwm thiab kev tswj hwm lub cev tsis muaj zog, 5-hydroxytryptamine (5-HT) cov nyhuv thiab qhov mob nyem.Tsis tas li ntawd, peb tau tham txog PRP cov txheej txheem ntsig txog kev mob o thiab angiogenesis thaum kho cov ntaub so ntswg thiab rov tsim dua tshiab.Thaum kawg, peb yuav tshuaj xyuas cov teebmeem ntawm qee cov tshuaj ntawm PRP kev ua haujlwm.
Autologous platelet-nplua nuj plasma (PRP) yog cov kua ib feem ntawm autologous peripheral ntshav tom qab kev kho mob, thiab cov platelet concentration yog siab tshaj qhov pib.PRP txoj kev kho tau siv rau ntau yam kev qhia rau ntau tshaj 30 xyoo, uas ua rau muaj kev txaus siab rau lub peev xwm ntawm autogenous PRP hauv cov tshuaj regenerative.Lub sij hawm orthopedic lom neeg sawv cev tsis ntev los no tau qhia los kho cov kab mob musculoskeletal (MSK), thiab tau ua tiav cov txiaj ntsig tau zoo hauv kev muaj peev xwm rov tsim dua ntawm heterogeneous bioactive PRP cell sib tov.Tam sim no, PRP txoj kev kho yog ib qho kev kho mob uas tsim nyog nrog cov txiaj ntsig kho mob, thiab cov ntaub ntawv qhia tus neeg mob tau txhawb nqa.Txawm li cas los xij, qhov tsis sib xws ntawm cov txiaj ntsig ntawm tus neeg mob thiab cov kev pom tshiab tau ua rau muaj teeb meem rau kev ua tau zoo ntawm kev siv tshuaj kho mob ntawm PRP.Ib qho ntawm cov laj thawj yuav yog tus lej thiab qhov sib txawv ntawm PRP thiab PRP-hom tshuab hauv khw.Cov khoom siv no sib txawv raws li PRP ntim ntim thiab cov txheej txheem npaj, ua rau PRP cov yam ntxwv tshwj xeeb thiab cov tshuaj lom neeg.Tsis tas li ntawd, qhov tsis muaj kev pom zoo ntawm tus qauv kev npaj ntawm PRP thiab daim ntawv qhia tag nrho ntawm cov tshuaj lom neeg hauv kev kho mob tau ua rau cov ntaub ntawv tsis sib xws.Ntau qhov kev sim tau ua los ua tus cwj pwm thiab cais PRP lossis cov khoom siv ntshav los ntawm cov ntawv thov tshuaj kho dua tshiab.Tsis tas li ntawd, platelet derivatives, xws li tib neeg platelet lysates, tau raug npaj rau kev tshawb fawb orthopedic thiab hauv vitro qia cell.
Ib qho ntawm thawj cov lus pom ntawm PRP tau luam tawm xyoo 2006. Lub hom phiaj tseem ceeb ntawm qhov kev tshuaj xyuas no yog kev ua haujlwm thiab hom kev ua ntawm platelets, cov nyhuv ntawm PRP ntawm txhua theem ntawm kev kho mob cascade, thiab lub luag haujlwm tseem ceeb ntawm platelet-derived kev loj hlob. nyob rau hauv ntau yam PRP indications.Nyob rau theem pib ntawm kev tshawb fawb PRP, qhov kev txaus siab tseem ceeb hauv PRP lossis PRP-gel yog qhov muaj thiab cov haujlwm tshwj xeeb ntawm ntau yam platelet loj hlob (PGF).
Nyob rau hauv daim ntawv no, peb yuav nthuav dav tham txog qhov kev txhim kho tshiab kawg ntawm cov qauv PRP particle sib txawv thiab platelet cell membrane receptors thiab lawv cov teebmeem ntawm innate thiab adaptive lub cev tiv thaiv kab mob.Tsis tas li ntawd, lub luag haujlwm ntawm tus kheej lub hlwb uas yuav muaj nyob hauv PRP kev kho mob vial thiab lawv cov kev cuam tshuam ntawm cov txheej txheem tsim cov ntaub so ntswg yuav raug tham kom ntxaws.Tsis tas li ntawd, qhov kev nce qib tshiab kawg hauv kev nkag siab txog PRP cov tshuaj lom neeg, koob tshuaj platelet, cov teebmeem tshwj xeeb ntawm cov qe ntshav dawb, thiab cov teebmeem ntawm PGF concentration thiab cytokines ntawm cov khoom noj muaj txiaj ntsig ntawm mesenchymal qia hlwb (MSCs) yuav raug piav qhia, suav nrog PRP lub hom phiaj sib txawv. cell thiab cov ntaub so ntswg ib puag ncig tom qab cell signal transduction thiab paracrine cuam tshuam.Ib yam li ntawd, peb yuav sib tham txog PRP cov txheej txheem cuam tshuam txog kev mob thiab kev mob angiogenesis thaum kho cov ntaub so ntswg thiab rov tsim dua tshiab.Thaum kawg, peb yuav tshuaj xyuas cov nyhuv analgesic ntawm PRP, cov nyhuv ntawm qee cov tshuaj ntawm PRP kev ua, thiab kev sib xyaw ntawm PRP thiab cov kev pabcuam kho mob.
Cov hauv paus ntsiab lus ntawm kev kho mob platelet-nplua nuj plasma therapy
Kev npaj PRP tau nrov zuj zus thiab siv dav hauv ntau yam kev kho mob.Lub hauv paus ntsiab lus tshawb fawb ntawm PRP kev kho mob yog tias kev txhaj tshuaj ntawm cov ntshav platelets ntawm qhov chaw raug mob tuaj yeem pib kho cov ntaub so ntswg, kev sib txuas ntawm cov ntaub so ntswg tshiab thiab kev tsim kho cov ntshav los ntawm kev tso tawm ntau yam khoom siv roj ntsha (kev loj hlob, cytokines, lysosomes) thiab adhesion proteins lub luag hauj lwm rau pib lub hemostatic cascade cov tshuaj tiv thaiv.Tsis tas li ntawd, plasma proteins (xws li fibrinogen, prothrombin, thiab fibronectin) muaj nyob rau hauv platelet-poor plasma Cheebtsam (PPPs).PRP mloog zoo tuaj yeem txhawb nqa qhov kev tso tawm hyperphysiological ntawm kev loj hlob yam tseem ceeb los pib kho cov kev raug mob ntev thiab ua kom cov txheej txheem kho mob hnyav.Nyob rau txhua theem ntawm cov txheej txheem kho cov ntaub so ntswg, ntau yam kev loj hlob, cytokines thiab cov tswj hwm hauv zos txhawb nqa feem ntau cov haujlwm ntawm tes los ntawm endocrine, paracrine, autocrine thiab endocrine mechanisms.Cov txiaj ntsig tseem ceeb ntawm PRP suav nrog nws txoj kev nyab xeeb thiab kev npaj siv thev naus laus zis ntawm cov khoom lag luam tam sim no, uas tuaj yeem siv los npaj cov tshuaj lom neeg uas tuaj yeem siv dav.Qhov tseem ceeb tshaj plaws, piv nrog cov tshuaj corticosteroids, PRP yog cov khoom lag luam autogenous uas tsis paub cov kev mob tshwm sim.Txawm li cas los xij, tsis muaj kev cai meej meej ntawm cov qauv thiab muaj pes tsawg leeg ntawm cov tshuaj PRP muaj pes tsawg leeg, thiab cov muaj pes tsawg leeg ntawm PRP muaj cov kev hloov pauv loj hauv platelets, cov ntsiab lus ntawm cov qe ntshav dawb (WBC), ntshav liab (RBC) cov pa phem, thiab PGF concentration.
PRP terminology thiab kev faib tawm
Tau ntau xyoo lawm, txoj kev loj hlob ntawm PRP cov khoom siv los txhawb cov ntaub so ntswg kho thiab rov tsim dua tshiab tau ua ib qho kev tshawb fawb tseem ceeb ntawm biomaterials thiab tshuaj science.Cov ntaub so ntswg kho cascade suav nrog ntau tus neeg koom nrog, suav nrog cov platelets thiab lawv cov kev loj hlob thiab cytokine granules, cov qe ntshav dawb, fibrin matrix thiab ntau lwm cov cytokines synergistic.Hauv cov txheej txheem cascade no, cov txheej txheem coagulation nyuaj yuav tshwm sim, suav nrog kev ua kom platelet ua kom sai thiab tom qab densification thiab α- tso tawm cov ntsiab lus ntawm platelet hais, kev sib sau ntawm fibrinogen (tso tawm los ntawm platelets lossis dawb hauv plasma) rau hauv fibrin network, thiab tsim. ntawm platelet embolism.
"Universal" PRP simulates pib kho
Thaum xub thawj, lo lus "platelet-nplua nuj ntshav plasma (PRP)" yog hu ua platelet concentrate siv hauv cov tshuaj ntshav, thiab nws tseem siv niaj hnub no.Thaum xub thawj, cov khoom PRP no tsuas yog siv los ua cov ntaub nplaum fibrin, thaum platelets tsuas yog siv los txhawb kev muaj zog fibrin polymerization los txhim kho cov ntaub so ntswg, es tsis yog kev kho mob.Tom qab ntawd, PRP thev naus laus zis tau tsim los simulate qhov pib ntawm kev kho mob cascade.Tom qab ntawd, PRP thev naus laus zis tau sau tseg los ntawm nws lub peev xwm los qhia thiab tso tawm qhov kev loj hlob mus rau hauv cheeb tsam microenvironment.Qhov kev txaus siab rau PGF xa khoom feem ntau zais lub luag haujlwm tseem ceeb ntawm lwm cov khoom hauv cov ntshav derivatives.Qhov kev txaus siab no ntxiv zuj zus ntxiv vim tsis muaj cov ntaub ntawv tshawb fawb, kev ntseeg mystical, kev nyiam ua lag luam thiab tsis muaj qauv thiab kev faib tawm.
Lub biology ntawm PRP concentrate yog complex raws li cov ntshav nws tus kheej, thiab tej zaum yuav nyuaj tshaj li ib txwm tshuaj.PRP cov khoom lag luam yog biomaterials.Cov txiaj ntsig ntawm kev kho mob PRP daim ntawv thov yog nyob ntawm tus yam ntxwv ntawm tus neeg mob, universal thiab adaptive yam ntxwv ntawm tus neeg mob cov ntshav, nrog rau ntau yam lwm yam cellular Cheebtsam uas tej zaum yuav muaj nyob rau hauv cov qauv PRP thiab lub zos microenvironment ntawm lub receptor, uas yuav nyob rau hauv mob los yog mob.
Cov ntsiab lus ntawm qhov tsis meej pem PRP cov ntsiab lus thiab cov txheej txheem kev faib tawm
Tau ntau xyoo, cov kws kho mob, cov kws tshawb fawb thiab cov tuam txhab lag luam tau cuam tshuam los ntawm kev nkag siab tsis meej thiab tsis zoo ntawm PRP cov khoom thiab lawv cov ntsiab lus sib txawv.Qee tus kws sau ntawv txhais tau tias PRP yog platelet nkaus xwb, thaum lwm tus tau taw qhia tias PRP tseem muaj cov qe ntshav liab, ntau cov qe ntshav dawb, fibrin thiab cov proteins uas muaj zog ntau ntxiv.Yog li ntawd, ntau yam sib txawv PRP tshuaj lom neeg tau raug qhia rau hauv kev kho mob.Nws yog kev poob siab tias cov ntaub ntawv feem ntau tsis muaj cov lus piav qhia ntxaws txog cov tshuaj lom neeg.Qhov tsis ua tiav ntawm kev npaj cov khoom lag luam thiab kev txhim kho kev faib tawm tom qab tau coj mus rau kev siv ntau ntawm PRP cov khoom piav qhia los ntawm cov ntsiab lus sib txawv thiab cov ntawv luv.Nws tsis yog qhov xav tsis thoob tias kev hloov pauv hauv PRP kev npaj ua rau cov neeg mob tsis sib haum xeeb.
Kingsley thawj zaug siv lo lus "platelet-nplua nuj plasma" nyob rau hauv 1954. Ntau xyoo tom qab, Ehrenfest li al.Thawj qhov kev faib tawm raws li peb qhov tseem ceeb (platelet, leukocyte thiab fibrin cov ntsiab lus) tau npaj tseg, thiab ntau PRP cov khoom tau muab faib ua plaub pawg: P-PRP, LR-PRP, ntshiab platelet-nplua nuj fibrin (P-PRF) thiab leucocyte. nplua nuj PRF (L-PRF).Cov khoom no yog npaj los ntawm tag nrho tsis siv neeg kaw system lossis phau ntawv raws tu qauv.Meanwhile, Everts et al.Qhov tseem ceeb ntawm kev hais txog cov qe ntshav dawb hauv kev npaj PRP tau hais meej.Lawv kuj pom zoo kom siv cov ntsiab lus tsim nyog los qhia txog qhov tsis ua haujlwm lossis qhib cov qauv ntawm PRP npaj thiab platelet gel.
Delong et al.npaj PRP kev faib tawm cov txheej txheem hu ua platelets, qhib cov qe ntshav dawb (PAW) raws li tus naj npawb ntawm cov platelets, suav nrog plaub lub platelet concentration ntau.Lwm yam tsis muaj xws li kev siv cov platelet activators thiab muaj lossis tsis muaj cov qe ntshav dawb (xws li neutrophils).Mishra et al.Ib qho kev faib tawm zoo sib xws yog npaj.Ob peb xyoos tom qab ntawd, Mautner thiab nws cov npoj yaig tau piav qhia txog qhov kev faib tawm kom ntxaws thiab ntxaws ntxaws (PLRA).Tus sau tau ua pov thawj tias nws yog ib qho tseem ceeb uas yuav tau piav qhia txog qhov tseeb platelet suav, cov ntsiab lus ntawm cov qe ntshav dawb (zoo lossis tsis zoo), feem pua neutrophil, RBC (zoo lossis tsis zoo) thiab seb puas siv exogenous activation.Nyob rau hauv 2016, Magalon et al.Kev faib tawm DEPA raws li cov koob txhaj tshuaj platelet, kev ua haujlwm ntau lawm, purity ntawm PRP tau txais thiab cov txheej txheem ua kom raug luam tawm.Tom qab ntawd, Lana thiab nws cov npoj yaig tau qhia txog MARSPILL kev faib tawm, tsom mus rau peripheral ntshav mononuclear hlwb.Tsis ntev los no, Pawg Neeg Saib Xyuas Kev Tshawb Fawb Txog Kev Tshawb Fawb tau tawm tswv yim rau kev siv cov txheej txheem kev faib tawm ntawm International Society for Thrombosis thiab Hemostasis, uas yog raws li cov lus pom zoo los ntawm ntau cov lus pom zoo los ua qauv siv cov khoom siv platelet hauv kev siv tshuaj kho dua tshiab, suav nrog cov khoom khov thiab thawed platelet.
Raws li PRP kev faib tawm cov txheej txheem npaj los ntawm ntau tus kws kho mob thiab cov kws tshawb fawb, ntau qhov kev sim ua tsis tiav los ua qauv tsim, txhais thiab cov qauv ntawm PRP siv los ntawm cov kws kho mob tuaj yeem txiav txim siab ncaj ncees, uas yuav tsis tshwm sim nyob rau ob peb xyoos tom ntej no ntxiv. , thev naus laus zis ntawm cov khoom siv kho mob PRP txuas ntxiv txhim kho, thiab cov ntaub ntawv tshawb fawb qhia tau tias kev npaj PRP sib txawv yog xav tau los kho cov kab mob sib txawv hauv cov xwm txheej tshwj xeeb.Yog li ntawd, peb cia siab tias qhov tsis muaj thiab qhov sib txawv ntawm qhov zoo tshaj plaws PRP ntau lawm yuav loj hlob mus ntxiv rau yav tom ntej.
Txoj kev npaj PRP tab tom ua tiav
Raws li PRP cov ntsiab lus thiab cov lus piav qhia ntawm cov khoom, ntau qhov kev faib tawm tau tso tawm rau cov qauv PRP sib txawv.Hmoov tsis zoo, tsis muaj kev pom zoo rau kev faib tawm dav dav ntawm PRP lossis lwm yam khoom siv ntshav thiab ntshav.Qhov zoo tshaj plaws, cov txheej txheem kev faib tawm yuav tsum tau them nyiaj rau ntau yam PRP cov yam ntxwv, cov ntsiab lus thiab cov npe tsim nyog cuam tshuam nrog kev txiav txim siab kho mob ntawm cov neeg mob tshwj xeeb.Tam sim no, daim ntawv thov orthopedic faib PRP ua peb pawg: ntshiab platelet-nplua nuj fibrin (P-PRF), leucocyte-nplua nuj PRP (LR-PRP) thiab leucocyte-tsis muaj PRP (LP-PRP).Txawm hais tias nws yog qhov tshwj xeeb tshaj li kev txhais cov khoom lag luam PRP, LR-PRP thiab LP-PRP pawg pom tseeb tsis muaj qhov tshwj xeeb hauv cov ntshav dawb.Vim nws lub cev tiv thaiv kab mob thiab kev tiv thaiv tus tswv cuab, cov qe ntshav dawb tau cuam tshuam zoo rau cov kab mob hauv nruab nrog cev ntawm cov kab mob ntev.Yog li, PRP cov tshuaj lom neeg muaj cov qe ntshav dawb tuaj yeem txhawb kev tiv thaiv kab mob thiab kho cov ntaub so ntswg thiab rov tsim dua tshiab.Tshwj xeeb tshaj yog, lymphocytes muaj ntau hauv PRP, tsim cov tshuaj insulin zoo li kev loj hlob thiab txhawb kev kho cov ntaub so ntswg.
Monocytes thiab macrophages ua lub luag haujlwm tseem ceeb hauv cov txheej txheem ntawm kev tiv thaiv kab mob thiab kev kho cov ntaub so ntswg.Qhov tseem ceeb ntawm neutrophils hauv PRP tsis meej.LP-PRP tau txiav txim siab ua thawj PRP kev npaj los ntawm kev ntsuam xyuas kom ua tiav cov txiaj ntsig kev kho mob zoo ntawm kev sib koom tes OA.Txawm li cas los xij, Lana et al.Kev siv LP-PRP hauv kev kho mob hauv caug OA tau tawm tsam, uas qhia tau hais tias cov qe ntshav dawb tshwj xeeb ua lub luag haujlwm tseem ceeb hauv cov txheej txheem inflammatory ua ntej cov ntaub so ntswg rov tsim dua tshiab, vim tias lawv tso cov tshuaj tiv thaiv kab mob thiab tiv thaiv kab mob.Lawv pom tias kev sib xyaw ntawm neutrophils thiab activated platelets muaj txiaj ntsig zoo dua li qhov tsis zoo ntawm kev kho cov ntaub so ntswg.Lawv kuj tau taw qhia tias plasticity ntawm monocytes yog ib qho tseem ceeb rau kev ua haujlwm tsis zoo thiab kho cov ntaub so ntswg.
Daim ntawv tshaj tawm ntawm PRP npaj tswv yim hauv kev tshawb fawb soj ntsuam yog qhov tsis sib xws.Feem ntau cov kev tshawb fawb luam tawm tsis tau npaj PRP npaj txoj kev xav tau rau kev rov ua dua ntawm cov tswv yim.Tsis muaj kev pom zoo meej ntawm kev kho mob, yog li nws nyuaj rau kev sib piv cov khoom PRP thiab lawv cov txiaj ntsig kev kho mob.Nyob rau hauv feem ntau cov ntaub ntawv qhia, platelet concentration kho yog cais nyob rau hauv lub sij hawm "PRP", txawm rau tib lub chaw kho mob qhia.Rau qee qhov kev kho mob (xws li OA thiab tendinosis), kev ua tiav tau ua tiav hauv kev nkag siab txog kev hloov pauv ntawm PRP kev npaj, kev xa khoom, platelet muaj nuj nqi thiab lwm yam PRP cov khoom uas cuam tshuam rau kev kho cov ntaub so ntswg thiab cov ntaub so ntswg.Txawm li cas los xij, kev tshawb fawb ntxiv yog xav tau kom ncav cuag qhov kev pom zoo ntawm PRP cov lus hais txog PRP cov tshuaj lom neeg txhawm rau txhawm rau txhawm rau kho qee yam kab mob thiab kab mob.
Cov xwm txheej ntawm PRP kev faib tawm qhov system
Kev siv autologous PRP biotherapy yog teeb meem los ntawm heterogeneity ntawm PRP kev npaj, tsis sib xws naming thiab tsis zoo standardization ntawm pov thawj-raws li cov lus qhia (uas yog, muaj ntau ntau txoj kev npaj los tsim tshuaj kho vials).Nws tuaj yeem kwv yees tias qhov tseeb PRP cov ntsiab lus, purity thiab lom cov yam ntxwv ntawm PRP thiab cov khoom muaj feem xyuam sib txawv heev, thiab cuam tshuam rau kev ua haujlwm lom neeg thiab kev sim tshuaj ntsuam xyuas.Kev xaiv ntawm PRP npaj cov cuab yeej qhia txog thawj qhov tseem ceeb sib txawv.Hauv kev kho cov tshuaj kho dua tshiab, cov kws kho mob tuaj yeem siv ob qho kev npaj PRP sib txawv thiab txoj hauv kev.Ib qho kev npaj siv cov txheej txheem ntshav cell cais, uas ua haujlwm ntawm cov ntshav ua tiav los ntawm nws tus kheej.Txoj kev no siv cov nruam ntws centrifuge nruas lossis disk sib cais tshuab thiab cov kauj ruam nyuaj thiab mos centrifuge.Feem ntau ntawm cov cuab yeej no yog siv hauv kev phais.Lwm txoj kev yog siv lub ntiajteb txawj nqus centrifugal technology thiab khoom siv.High G-force centrifugation yog siv los cais cov txheej daj ntawm ESR los ntawm cov ntshav uas muaj platelets thiab cov qe ntshav dawb.Cov khoom siv concentration no me dua li cov qe ntshav sib cais thiab tuaj yeem siv ib sab ntawm lub txaj.Hauv qhov sib txawv ģ - Lub sijhawm quab yuam thiab centrifugation ua rau muaj qhov sib txawv tseem ceeb hauv kev tawm los, kev xav, kev coj dawb huv, kev muaj peev xwm, thiab lub xeev ua haujlwm ntawm cov platelets cais.Ntau hom kev lag luam PRP npaj cov cuab yeej siv tau rau hauv pawg tom kawg, uas ua rau muaj kev hloov pauv ntxiv hauv cov ntsiab lus ntawm cov khoom.
Qhov tsis muaj kev pom zoo ntawm txoj kev npaj thiab kev siv tau ntawm PRP tseem ua rau muaj kev tsis sib haum xeeb ntawm PRP kev kho mob, thiab muaj qhov sib txawv loj hauv PRP kev npaj, cov qauv zoo thiab cov txiaj ntsig kev kho mob.Cov khoom lag luam PRP uas twb muaj lawm tau raug txheeb xyuas thiab sau npe raws li cov lus qhia tshwj xeeb ntawm cov chaw tsim khoom, uas daws qhov sib txawv ntawm cov khoom siv PRP tam sim no.
Nkag siab platelet koob tshuaj hauv vitro thiab hauv vivo
Cov nyhuv kho ntawm PRP thiab lwm yam platelet concentrates stems los ntawm kev tso tawm ntau yam cuam tshuam nrog kev kho cov ntaub so ntswg thiab rov tsim dua tshiab.Tom qab ua kom cov platelets, platelets yuav tsim platelet thrombus, uas yuav ua hauj lwm raws li ib ntus extracellular matrix los txhawb cell proliferation thiab sib txawv.Yog li ntawd, nws yog qhov ncaj ncees rau kev xav tias cov koob tshuaj platelet ntau dua yuav ua rau muaj qhov siab dua hauv zos ntawm platelet bioactive yam.Txawm li cas los xij, kev sib raug zoo ntawm cov koob tshuaj thiab cov concentration ntawm cov platelets thiab cov concentration ntawm platelet bioactive kev loj hlob ntawm cov tshuaj thiab tshuaj tej zaum yuav tswj tsis tau, vim hais tias muaj qhov sib txawv tseem ceeb ntawm cov platelet suav nruab nrab ntawm cov neeg mob, thiab muaj qhov sib txawv ntawm PRP txoj kev npaj.Ib yam li ntawd, ntau yam kev loj hlob ntawm platelet koom nrog hauv cov txheej txheem kho cov ntaub so ntswg muaj nyob rau hauv cov ntshav plasma ntawm PRP (piv txwv li, kev loj hlob ntawm daim siab thiab insulin zoo li kev loj hlob 1).Yog li ntawd, koob tshuaj platelet ntau dua yuav tsis cuam tshuam rau kev kho lub peev xwm ntawm cov kev loj hlob no.
Kev tshawb fawb hauv vitro PRP yog qhov nrov heev vim tias qhov sib txawv ntawm cov kev tshawb fawb no tuaj yeem tswj tau thiab cov txiaj ntsig tau sai.Ntau qhov kev tshawb fawb tau pom tias cov hlwb teb rau PRP raws li koob tshuaj.Nguyen thiab Pham tau pom tias muaj ntau ntau ntawm GF tsis tas yuav ua rau cov txheej txheem ntawm tes stimulation, uas tej zaum yuav muaj kev cuam tshuam.Qee qhov kev tshawb fawb hauv vitro tau pom tias qhov siab PGF siab tuaj yeem muaj kev cuam tshuam tsis zoo.Ib qho laj thawj yuav yog qhov tsawg ntawm cov cell membrane receptors.Yog li ntawd, ib zaug PGF qib siab dhau los piv nrog cov receptors muaj, lawv yuav muaj kev cuam tshuam tsis zoo rau kev ua haujlwm ntawm tes.
Qhov tseem ceeb ntawm platelet concentration cov ntaub ntawv hauv vitro
Txawm hais tias kev tshawb fawb hauv vitro muaj ntau qhov zoo, nws kuj muaj qee qhov tsis zoo.Hauv vitro, vim muaj kev cuam tshuam tsis tu ncua ntawm ntau hom cell sib txawv hauv cov ntaub so ntswg vim yog cov qauv ntawm cov ntaub so ntswg thiab cov ntaub so ntswg ntawm tes, nws yog qhov nyuaj rau rov ua dua hauv vitro hauv ob qho tib si kab lis kev cai ib puag ncig.Cov xovtooj ntawm tes uas tuaj yeem cuam tshuam rau txoj kev teeb liab ntawm tes yog feem ntau tsawg dua 1% ntawm cov ntaub so ntswg.Ob-dimensional kab lis kev cai tais cov ntaub so ntswg tiv thaiv cov hlwb los ntawm raug rau extracellular matrix (ECM).Tsis tas li ntawd, cov kab lis kev cai niaj hnub siv tshuab yuav ua rau cov khoom siv ntawm tes khib nyiab thiab noj cov khoom noj tsis tu ncua.Yog li ntawd, nyob rau hauv vitro kab lis kev cai yog txawv los ntawm ib qho kev mob nyob rau hauv lub xeev, cov ntaub so ntswg oxygen mov los yog tam sim ntawd pauv ntawm kab lis kev cai nruab nrab, thiab tsis sib haum xeeb tshwm sim tau luam tawm, muab piv cov kev kho mob nyhuv ntawm PRP nrog in vitro txoj kev tshawb no ntawm cov hlwb, cov ntaub so ntswg hom thiab platelet. concentrations.Graziani thiab lwm tus.Nws tau pom tias hauv vitro, qhov cuam tshuam loj tshaj plaws ntawm kev loj hlob ntawm osteoblasts thiab fibroblasts tau ua tiav ntawm PRP platelet concentration 2.5 npaug ntau dua li tus nqi hauv paus.Hauv qhov sib piv, cov ntaub ntawv kho mob tau muab los ntawm Park thiab cov npoj yaig tau pom tias tom qab kev sib koom ua ke ntawm tus txha caj qaum, PRP platelet theem yuav tsum tau nce ntau dua 5 npaug tshaj li cov hauv paus ntsiab lus los txhawb cov txiaj ntsig zoo.Cov txiaj ntsig zoo sib xws kuj tau tshaj tawm ntawm cov ntaub ntawv kev loj hlob ntawm cov leeg hauv vitro thiab cov txiaj ntsig kho mob.
(Cov ntsiab lus ntawm tsab xov xwm no tau luam tawm dua, thiab peb tsis muab ib qho kev qhia lossis kev lees paub rau qhov raug, kev ntseeg tau lossis ua tiav ntawm cov ntsiab lus muaj nyob rau hauv tsab xov xwm no, thiab tsis muaj lub luag haujlwm rau cov kev xav ntawm tsab xov xwm no, thov nkag siab.)
Post lub sij hawm: Mar-01-2023